REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
APC anti-mouse CD45.1 Antibody	BioLegend	Cat#110714; RRID:AB_313503
FITC anti-mouse CD3 Antibody	BioLegend	Cat#100204; RRID:AB_312661
PE anti-mouse CD4 Antibody	BioLegend	Cat#100408; RRID:AB_312693
FITC anti-mouse CD4 Antibody	BioLegend	Cat#100406; RRID:AB_312691
PerCP/Cyanine5.5 anti-mouse CD8a Antibody	BioLegend	Cat#100734; RRID:AB_2075238
FITC anti-mouse CD11b Antibody	BioLegend	Cat#101206; RRID:AB_312789
PE anti-mouse CD86 Antibody	BioLegend	Cat#159203; RRID:AB_2832567
PE/Cyanine7 anti-mouse CD11c Antibody	BioLegend	Cat#117318; RRID:AB_493568
PE anti-mouse IFN-γ Antibody	BioLegend	Cat#163504; RRID:AB_2890730
PE anti-mouse FOXP3 Antibody	BioLegend	Cat#126404; RRID:AB_1089117
PE/Cyanine7 anti-mouse F4/80 Antibody	BioLegend	Cat#123114; RRID:AB_893478
PE/Cyanine7 anti-mouse CD69 Antibody	BioLegend	Cat#104512; RRID:AB_493564
PerCP/Cyanine5.5 anti-mouse CD206 (MMR) Antibody	BioLegend	Cat#141716; RRID:AB_2561992
PE anti-mouse CD49b Antibody	BioLegend	Cat#103506; RRID:AB_313029
PerCP/Cyanine5.5 anti-mouse CD25 Antibody	BioLegend	Cat#101912; RRID:AB_10613643
PE/Cyanine7 anti-mouse/human Ki67 Antibody	BioLegend	Cat#151217; RRID:AB_2910305
PerCP/Cyanine5.5 anti-human CD206 (MMR) Antibody	BioLegend	Cat#321122; RRID:AB_10899411
PE anti-human CD86 Antibody	BioLegend	Cat#381010; RRID:AB_3083375
InVivoMAb anti-mouse CD8a	BioXCell	Cat#BE0061; RRID:AB_1125541
InVivoMAb anti-mouse CSF1R(CD115)	BioXCell	Cat#BE0213; RRID:AB_2687699
6x-His Tag Monoclonal Antibody	Invitrogen	Cat#4E3D10H2/E3; RRID:AB_2536841
Goat anti-Mouse IgG (H + L) Secondary Antibody, HRP	Invitrogen	Cat#31430; RRID:AB_228307
Biological samples
Human PBMC	Blood from the healthy donor	N/A
Human TNBC tumor	Tumor sample from the TNBC patients in Nanjing Drum Tower Hospital	N/A
Chemicals, proteins, and recombinant proteins
Dulbecco’s modified Eagle’s medium (DMEM)	Invitrogen	Cat#10564011
Fetal Bovine Serum (FBS)	Invitrogen	Cat#A4766801
Streptomycin/penicillin	Invitrogen	Cat#15140122
Opti-MEM	Invitrogen	Cat#31985070
Roswell Park Memorial Institute (RPMI) 1640	Invitrogen	Cat#21875034
293 Pro	BasalMedia	Cat#F431166
Foxp3 fixation/permeabilization buffer	eBioscience	Cat#00-5523-00
Permeabilization kit	eBioscience	Cat#00-5523-00
DNAse I	Roche	Cat#10104159001
Collagenase A	Roche	Cat#10103586001
Lipofectamine 2000	Invitrogen	Cat#11668019
GeltrexTM Basement Membrane Matrix	Invitrogen	Cat#A1413202
Perteinase K Solution	Invitrogen	Cat#25530049
Kananmycin	Invitrogen	Cat#11-815-024
Ampicillin	Bioreagents	Cat#BP1760-25
Critical commercial assays
HiScript Reverse Transcriptase reagent kit	Vanzyme	Cat#R101-02
HiScript II One Step RT-PCR Kit	Vanzyme	Cat#P611-01
Adeno-XTM rapid titer kit	Clontech	Cat#632250
CD4+ T cell isolation kit, mouse	Selleck	Cat#B90000
CD8+ T cell isolation kit, mouse	Selleck	Cat#B90011
Mouse IL-1β ELISA kit	Elabscience	Cat#E-EL-M0046c
Human IL-1β ELISA kit	Elabscience	Cat#E-EL-H0149
Mouse IL-10 ELISA kit	Elabscience	Cat#E-EL-H0149c
Human IL-10 ELISA kit	Elabscience	Cat#E-EL-H6154
Deposited data
4T1 cells RNA sequencing	GEO database	GEO: GSE271046
Macrophages RNA sequencing	GEO database	GEO: GSE271202
Experimental models: Cell lines
4T1 cells	ATCC	Cat#CRL-2537
CT26 cells	ATCC	Cat#CRL-2638
H22 cells	This paper	N/A
B16-F10	ATCC	Cat#CRL-6475
MDA-MB-231 cells	ATCC	Cat#CRM-HTB-26
HCT116 cells	ATCC	Cat#CCL-247EMT
HepG2 cells	ATCC	Cat#HB-8065
MET-5A cells	ATCC	Cat#CRL-9444
THP-1 cells	ATCC	Cat#TIB-202
HEK 293T cells	ATCC	Cat#CRL-1573
RAW264.7 cells	ATCC	Cat#TIB-71
Experimental models: Organisms/strains
Mouse: BALB/c	GemPharmatech	SN#000651
Mouse: Outbred athymic nude	GemPharmatech	SN#007850
Mouse: C57BL/6J	GemPharmatech	SN#000664
Mouse: CD11c-dtr B6.FVB-1700016L21RikTg(Itgax−HBEGF/EGFP)57Lan/J	Jackson Laboratory	SN#004509
Mouse: NCG mice NOD/ShiLtJGpt-Prkdcem26Cd52Il2rgem26Cd22/Gpt	GemPharmatech	SN#T001475
DH5α	Vanzyme	Cat# C502-02
GFP+Escherichia coli	This paper	N/A
Oligonucleotides
See Table S1 for primers	N/A	N/A
Recombinant DNA
pAd-DEST	This paper	N/A
pAd-Tα1	This paper	N/A
Software and algorithms
GraphPad Prism 9	N/A	N/A
FlowJo 10.7.1	Tree Star	N/A
Adobe Illustrator	Adobe	N/A
BioRender	BioRender	N/A