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. 2024 Oct 15;5(10):101788. doi: 10.1016/j.xcrm.2024.101788

Figure 3.

Figure 3

The genes encoding NaV1.8 and NaV1.9 show different expression levels in OPRM1-positive and OPRM1-negative nociceptors

(A) Representative section of human DRG showing neurons positive for TRPV1, the μ-opioid receptor (OPRM1), and voltage-gated sodium channels NaV1.8 (SCN10A) and NaV1.9 (SCN11A) transcripts. Representative neurons characterized further in (E) and (F) are labeled with small Roman numerals.

(B) Enlarged field outlined in (A) showing each individual transcript. Overlap of all four transcripts occurs in a substantial subpopulation (i). Lipofuscin is marked with an “L.”

(C) Percentage of 1,310 neurons expressing each individual transcript.

(D) Percentage of neurons expressing the most common transcript combinations. Bar graphs in (C) and (D) show mean, SD, and individual values from four independent donors.

(E) Multi-channel microscopy images of a representative individual neuron of each population and the population’s cell size distribution. Scale bars, 25 μm.

(F) Expression intensity of individual transcripts in OPRM1-positive (i) as compared to OPRM1-negative (ii) nociceptors. Transcript levels for TRPV1 and SCN11A were significantly higher in the OPRM1-negative population. Median and interquartile range indicated. p < 0.001, Mann-Whitney U test.