| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| guinea pig anti-EndoA (GP69) | Verstreken et al.58 | N/A |
| rabbit anti-GFP | Invitrogen | Cat#: A11122; RRID: AB_221569 |
| goat anti-GFP | Abcam | Cat#: ab5450; RRID: AB_304897 |
| mouse anti-Brp | DSHB | Cat#: nc82; RRID: AB_2314866 |
| mouse anti-Syntaxin1A | DSHB | Cat#: 8C3; RRID: AB_528484 |
| mouse anti-CSP | DSHB | Cat#: ab49; RRID: AB_2307345 |
| mouse anti-GluRIIA | DSHB | Cat#: 8B4D2; RRID: AB_528269 |
| mouse anti-Synaptotagmin1 | DSHB | Cat#:3H2 2D7; RRID: AB_528483 |
| rabbit anti-Synaptojanin | Verstreken et al.56 | N/A |
| guinea pig anti-Vha100-1 | Gift from Robin Hiesinger71 | N/A |
| mouse anti-Synapsin | DSHB | Cat#: 3C11; RRID: AB_528479 |
| mouse anti-Dynamin | DB Biosciences | Cat#: 610246; RRID: AB_397641 |
| rat anti-Synaptobrevin | Gift from Hugo Bellen | N/A |
| mouse anti-DLG | DSHB | Cat#: 4F3; RRID: AB_528203 |
| rabbit anti-Tyrosine Hydroxylase (TH) | Millipore | Cat#: AB152; RRID: AB_390204 |
| rabbit anti-GAPDH | Thermo Fisher | Cat#: PA1-16777; RRID: AB_568552 |
| rabbit anti-HRP | Jackson Immuno Research | Cat#: 323-005-021; RRID: AB_2314648 |
| Alexa Fluor 488 goat anti-rabbit | Invitrogen | Cat#: A11034; RRID: AB_2576217 |
| Alexa Fluor 555 goat anti-rabbit | Invitrogen | Cat#: A27039; RRID: AB_2536100 |
| Alexa Fluor 647 goat anti-mouse | Invitrogen | Cat#: A21236; RRID: AB_2535805 |
| Alexa Fluor 555 goat anti-mouse | Invitrogen | Cat#: A21422; RRID: AB_141780 |
| Alexa Fluor 647 goat anti-guinea pig | Invitrogen | Cat#: A21450; RRID: AB_2735091 |
| Alexa Fluor 488 goat anti-rat | Invitrogen | Cat#: A11006; RRID: AB_2534074 |
| Alexa Fluor 647 anti-goat | Invitrogen | Cat#: A21447; RRID: AB_2535864 |
| Chemicals, peptides, and recombinant proteins | ||
| FM1–43 Dye (N-(3-Triethylammoniumpropyl)-4-(4-(Dibutylamino) Styryl) Pyridinium Dibromide) | Invitrogen | Cat#: T3163 |
| Critical commercial assays | ||
| Maxwell RSC microRNA tissue kit | Promega | Cat#: AS1460 |
| SuperScript III First-Strand Synthesis System for RT-PCR | Thermo Fisher | Cat#: 18080051 |
| Quick Start Bradford Protein Assay kit | Bio-Rad | Cat#: 5000202 |
| Ion Ampliseq Exome Kit | Thermo Fisher | Cat#: A38264 |
| CytoScan™ HD Array Kit | Thermo Fisher | Cat#: 901835 |
| Ampli Taq Gold Fast PCR Master | Thermo Fisher | Cat#: 4390941 |
| Big Dye v3.1 Cycle Sequencing kit | Thermo Fisher | Cat#: 4337455 |
| Experimental models: organisms/strains | ||
| D. melanogaster: w1118 | BDSC | RRID: BDSC_3605; Fly base: FBal0018186 |
| D. melanogaster: GMR57C10-Gal4 | BDSC | RRID: BDSC_39171; Fly base: FBti0137043 |
| D. melanogaster: cn bw | BDSC | RRID: BDSC_264; Fly base: FBst0000264 |
| D. melanogaster: UAS-Evi-GFP | Gift from Michael Boutrons72 | N/A |
| D. melanogaster: UAS-DF-Syt1 | Gift from Robin Hiesinger73 | N/A |
| D. melanogaster: y[1] w[67c23]; Mi{PT-GFSTF.2}VGlut[MI04979-GFSTF.2] | BDSC74 | RRID: BDSC_59411 |
| D. melanogaster: CG8176- | This study | N/A |
| D. melanogaster: CG8176 WT | This study | N/A |
| D. melanogaster: UAS-GFP-CG8176 WT | This study | N/A |
| D. melanogaster: UAS-GFP-CG8176 WG | This study | N/A |
| Fly lines used in this study, see Table S4 | – | N/A |
| Oligonucleotides | ||
| Primers for RT-PCR, see Table S4 | This study | N/A |
| Primers for cloning, see Table S4 | This study | N/A |
| Homology arms, see Table S4 | This study | N/A |
| gBlocks for cloning, see Table S4 | This study | N/A |
| Recombinant DNA | ||
| pCFD4 | Gift from Simon Bullock75 | RRID: Addgene_49411 |
| pCFD4_gRNA | This study | N/A |
| pWhite-STAR | Choi et al.76 | N/A |
| pWhiteSTAR_dSgip1 | This Study | N/A |
| pUC19 | Addgene | RRID:Addgene_50005 |
| pReC_dSgip1-WT | This Study | N/A |
| pUAST attB w+ | Bischof at al.77 | N/A |
| pUAST-GFP-dSgip1-WT | This Study | N/A |
| pUAST-GFP-dSgip1-WG | This Study | N/A |
| Software and algorithms | ||
| GraphPad Prism 9 | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| ImageJ-win64 | National Institute of Health | https://imagej.nih.gov/ij/ |
| Inkscape 1.1.2 | Inkscape’s Contributors | https://inkscape.org/de/release/inkscape-1.1.2/ |
| NIS-Elements | Nikon | https://www.microscope.healthcare.nikon.com/ |
| Zen black | Zeiss | https://www.zeiss.com/ |
| Axoscope 10.7 | Molecular Devices | https://support.moleculardevices.com/s/article/Molecular-Devices-Software |
| Igor Pro 6.37 | Wave Metrics | https://www.wavemetrics.com/ |
| Axoclamp900A | Molecular Devices | https://support.moleculardevices.com/s/article/Molecular-Devices-Software |
| Clampfit 10.7 | Molecular Devices | https://support.moleculardevices.com/s/article/Molecular-Devices-Software |
| R Studio 3.6.3 | The R Project | https://www.r-project.org/ |
| GIMP 2.10.30 | GIMP | https://www.gimp.org/ |
| CLC workbench 22 | Qiagen | https://digitalinsights.qiagen.com/products/clc-main-workbench-direct-download/ |
| LAS v4.0 | Leica | https://www.leica-microsystems.com/ |
| LightCycler 480 Software | Roche | https://diagnostics.roche.com/ |
| Statistical Parametric Mapping | Functional Imaging Laboratory | https://www.fil.ion.ucl.ac.uk/spm/ |
| MATLAB 7.3 | MathWorks | https://www.mathworks.com/?s_tid=gn_logo |
| ScanVP | Feinstein Institutes for Neuroscience | https://feinsteinneuroscience.org/ |
| Ion Torrent Suit | Thermo Fisher | https://www.thermofisher.com/it/en/home/life-science/sequencing/next-generation-sequencing/ion-torrent-next-generation-sequencing-workflow/ion-torrent-next-generation-sequencing-data-analysis-workflow/ion-torrent-suite-software.html |
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