REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
IMPDH Antibody (F-6)	Santa Cruz	Cat#sc-166551; RRID:AB_2127354
mouse anti-GAPDH	Abcam	Cat#ab8245; RRID:AB_2107448
APC-conjugated anti-human TRA-1-85 (CD147)	Miltenyi Biotec	Cat#130-128-900; RRID:AB_2921968
HRP conjugated secondary anti mouse	BioRad	Cat#1706516; RRID:AB_11125547
Bacterial and virus strains
NEB Stable Competent E. coli	NEB	C3040
Biological samples
MDA-MB-231	ATCC	Cat#HTB-26
Normal human astrocytes	Lonza Bioscience	Discontinued
HEK293T	ATCC	Cat#CRL-11268
Chemicals, peptides, and recombinant proteins
Mycophenlic acid	Tocris	Cat#1505
PrestoBlue Cell Viability Reagent	ThermoFisher Scientific	Cat#A13261
SYTOX™ Green Nucleic Acid Stain	Invitrogen	Cat#S7020
Incucyte® Nuclight Rapid Red Dye for Live-Cell Nuclear Labeling	Sartorius	Cat#4717
7AAD Viability dye	Beckman Coulter	Cat#A07704
Liberase Blendzyme 3	Millipore Sigma	Cat#5401119001
Heparin Solution		Cat#07980
Human Recombinant Basic Fibroblast Growth Factor (bFGF)	STEMCELL Technologies	Cat#78003
Human Recombinant Epidermal Growth Factor (EGF)	STEMCELL Technologies	Cat#78006
Antibiotic-Antimycotic (100X)	Wisent Bioproducts	Cat#450-115-EL
Lipofectamine 3000	ThermoFisher	Cat#L3000075
Fetal Bovine Serum, heat inactivated (FBS)	Wisent	Cat#098-150
PBS	ThermoFisher	Cat#10010049
DMEM	ThermoFisher	Cat#11995073
EDTA	Millipore Sigma	Cat#20158
Guanine	Sigma-Aldrich	Cat#G11950
Paraformaldehyde	Electron Microscopy Biosciences	Cat#RT15700
D-firefly luciferin potassium salt	Perkin Elmer	Cat#122799
Critical commercial assays
Bradford Assay	BioRad	Cat#5000112
Deposited data
Lung-brain metastasis RNA seq data	Gene Expression Omnibus	GSE110495
Breast- and melanoma-brain metastasis RNA seq data	Gene Expression Omnibus	GSE220156
Metabolomics data	FigShare	https://doi.org/10.6084/m9.figshare.22246258
Experimental models: Cell lines
Patient-dervied BM lines	This manuscript	N/A
Neural Stem Cells	This manuscript	N/A
CRUK0748, CRUK0733	Provided by Prof. Charles Swanton	N/A
Experimental models: Organisms/strains
NOD-scid IL2Rgammanull (NSG) Mouse	The Jackson Laboratory	RRID:IMSR_JAX:005557
Recombinant DNA
pMD2.G	Addgene	Cat#12259; RRID:Addgene_12259
psPAX2	Addgene	Cat#12260; RRID:Addgene_12260
Toronto KnockOut (TKO) CRISPR Library - Version 3	Addgene	Cat#:90294; RRID:Addgene_52961
Firefly Luciferase	Addgene	RRID:Addgene_118017
Software and algorithms
Image Scope	Aperio	https://aperio-imagescope.software. informer.com
Living Image	Perkin Elmer	https://www.perkinelmer.com/es/lab-products-and-services/resourcesin-vivo-imaging-software-downloads.html
R	The R project for Statistical Computing	https://www.r-project.org/
GraphPad Prism	Graph Pad	https://www.graphpad.com/
ImageJ	NIH	https://imagej.nih.gov/
FlowJo v10.8	FlowJo LLC	https://www.flowjo.com/
BioRender	BioRender	https://biorender.com/
ELDA: Extreme Limiting Dilution Analysis	Walter+Eliza Hall Bioinformatics -Insitute of Medical Research	https://bioinf.wehi.edu.au/software/elda/
Aperio ScanScope	Leica Biosystems	N/A
FLUOstar Omega Fluorescence 556 Microplate reader	BMG labtech	N/A
Other
10 mL Hamilton syringe	Hamilton	Cat#76350-01
NeuroCult NS-A Proliferation Kit	Stem Cell Technologies	Cat#05751