REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Biological samples | ||
Human feces | This paper | N/A |
Critical commercial assays | ||
E.Z.N.A.® Soil DNA Kit | Omega Bio-tek, Norcross, GA, U.S. | Cat# D5625-01 |
Deposited data | ||
ITS2 rRNA sequencing data | This paper | Genome Sequence Archive (GSA): CRA013806 |
Metadata | This paper | https://www.cpc.unc.edu/projects/china/ |
Oligonucleotides | ||
ITS2 rRNA Forward Primer GCATCGATGAAGAACGCAGC |
Op De et al.34 | ITS3F |
ITS2 rRNA Reverse Primer TCCTCCGCTTATTGATATGC |
Op De et al.34 | ITS4R |
Software and algorithms | ||
Stata package v15.0 | StataCorp LLC, TX, USA | https://www.stata.com |
NetCoMi R package v.1.0.2 | Peschel et al.35 | https://github.com/stefpeschel/NetCoMi?tab=readme-ov-file |
vegan R package v.2.5.7 | CRAN | https://cran.r-project.org/web/packages/vegan/index.html |
microbiome R package v.1.19.1 | bioconductor | https://www.bioconductor.org/packages/release/bioc/html/microbiome.html |
lightgbm Python package v.3.2.1 | sklearn | https://lightgbm.readthedocs.io/en/stable/ |
ropls R package v.1.26.4 | bioconductor | https://bioconductor.org/packages/release/bioc/html/ropls.html |
WGCNA R package v.1.70.3 | CRAN | https://cran.r-project.org/web/packages/WGCNA/index.html |
mediation R package v.4.5.0 | CRAN | https://cran.r-project.org/web/packages/mediation/index.html |
QIIME 2 | Bolyen et al.15 | https://qiime2.org/ |
The computer code for main analyses | This paper |
https://github.com/wenutrition/CHNS-fungi-cardiometabolic-diseases Zenodo: https://doi.org/10.5281/zenodo.13353503 |