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. 2024 Oct 28;8(11):e70030. doi: 10.1002/hem3.70030

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© 2024 The Author(s). HemaSphere published by John Wiley & Sons Ltd on behalf of European Hematology Association.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Detection and quantification of circulating erythroid cells retaining mitochondria by flow cytometry in SS, SC patients, and AA healthy individuals. (A) Flow cytometry gating strategy of CD71/TO and/or MitoTracker Deep Red (MTKDR) staining. (B) Percentage of total (i) and stress (ii) reticulocytes in SS, SC patients, and AA healthy individuals and total (iii) and stress (iv) reticulocytes in SS patients treated or not with HU. (C) Percentage of mitochondria retention in total (i) and stress (ii) reticulocytes in SS, SC patients, and AA healthy individuals and in total (iii) and stress (iv) reticulocytes in SS patients treated or not by HU. (D) Mitochondria retention in mature RBCs in SS and SC patients and in AA controls (i) and in SS patients treated or not with HU (ii).