FIG. 6.

gE/gI accumulates in the TGN early after infection with wild-type HSV-1 but moves to cell junctions late in the infection. HEC-1A cells were infected with wild-type HSV-1 or with F-US7kan, a gI-negative HSV mutant. After 5 or 11 h, the cells were fixed, permeabilized, incubated with blocking buffer, and then incubated with sheep anti-TGN46 (red) for 1 h. The cells were then washed and incubated with donkey anti-sheep IgG coupled to Alexa 594. The cells were washed, blocked with 2% goat serum and 2% BSA, and then incubated simultaneously with a rat polyclonal serum specific for gE/gI (green) and rabbit serum specific for β-catenin (blue). The cells were washed and incubated with Alexa 488-conjugated goat anti-rat IgG antibodies and CY5-conjugated goat anti-rabbit IgG antibodies for 1 h. The cells were viewed by laser scanning confocal microscopy. The red, green, and blue channels were superimposed in the right panels (All markers).