FIG. 1.

Schematic representation of plasmids pPC1 and pPC2 and derived plasmids. (A) pPC1 and pPC2, from which T1 and T2 are obtained. Restriction sites used for the different constructions are indicated. Numbers correspond to the nucleotides' positions in the RNA. Arrow indicates readthrough. (B) pPC1 mutants expressing EGFP fused to either the N or C terminus of P15 and chimeric plasmids expressing P15 or P15 fused to EGFP. 5NC2, 5′ noncoding region from RNA-2; 3NC1, 3′ noncoding region from RNA-1.