FIG. 5.

Detection of CP in protoplasts inoculated with wild-type or mutant T1 and T2. Protoplasts (10⁶) 48 hpi were sedimented and resuspended in the loading buffer. Then a 10-μl aliquot, diluted as indicated below, was electrophoresed on an SDS–10% polyacrylamide gel. After transfer, CP was detected by enhanced chemiluminescence using a specific CP antiserum. Comparison of the intensity of the bands, evaluated by scanning, was used to estimate the amount of CP present in the different samples. The relative amount of CP present in the samples infected with mutant RNA-1 to that present in the samples infected with wild-type RNA-1 was calculated (column 1). Column 2 gives the relative amounts of progeny RNA-2 present in the same samples analyzed by Northern blot and quantified as in Table 1.