FIG. 8.

Localization of incorporated BrUTP and EGFP/P15 fusion protein. The two first rows show images from two representative T1-EG15 + T2-infected protoplasts (A to C and D to F) that were harvested 20 hpi, labeled for 6 h with BrUTP, fixed, and processed for immunofluorescence observation using antibodies that detect BrUTP. The last row shows mock-inoculated protoplasts processed identically (G and H). For each protoplast, green fluorescence images of EGFP/P15 (A, D, and G) and red fluorescence images of incorporated BrUTP (B, E, and H) were collected from the same 0.45-μm-thickness optical section with the multitrack confocal microscope and appropriate filters. The two images were digitally superimposed (C and F). Bar, 10 μm.