TABLE 2.
Amounts of positive- and negative-strand RNA-1 and RNA-2 relative to the wild-type RNAs detected in infected protoplasts at 48 hpi
| Inoculum | Progenya | % RNA detected
|
|
|---|---|---|---|
| Positive | Negative | ||
| T1-15(−) | RNA-1 | 21.3 | 47.6 |
| T1-15(−) + T2 | RNA-1 | 23.0 | 52.0 |
| RNA-2 | 24.0 | 49.0 | |
| T1-15Nh | RNA-1 | 17.3 | 41.5 |
| T1-15Nh + T2 | RNA-1 | 28.0 | 79.0 |
| RNA-2 | 23.0 | 66.0 | |
| T1-15LK | RNA-1 | 26.3 | 66.2 |
| T1-15LK + T2 | RNA-1 | 9.7 | 48.0 |
| RNA-2 | 30.0 | 58.0 | |
a
Progeny RNAs were detected using a probe corresponding to the 3′-terminal 124 nucleotides of RNA-1 for detection of negative strands of both RNA-1 and -2 and to its complement for the detection of the positive strands.