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. 2024 Oct 26;14:25555. doi: 10.1038/s41598-024-76482-y

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — SSCs are a discrete epidermal cell type that express classical airway GC markers. (a–b) SSC (red) and GC (blue) lineage clusters in X. tropicalis embryos at NF14 and from NF14-22, are discrete (a) and marked by lineage-specific expression of met and itln1, respectively (b). The canonical goblet cell markers, foxa1 and spdef, are expressed almost exclusively in the SSC cell cluster at NF14, and over developmental time (b). Scale bars: colour intensity represents normalised expression level from undetectable (0) to maximum (1) within the dataset. c. Chromogenic in situ hybridisation for spdef at NF22 reveals punctate expression throughout the skin (inset), typical of SSC distribution, and strong expression in the cement gland (asterisk). (d–f) Fluorescent in situ hybridisation of spdef mRNA (d) and dual staining with PNA (e) confirms that spdef is expressed in SSCs (merged in f).