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. 2024 Oct 27;14:25608. doi: 10.1038/s41598-024-76479-7

Fig. 1.

Fig. 1

(A) Alignment of nucleotide sequence for target Pvmdr 1 gene (Accession number AY571984.1). Arrows indicate sequences of designed F3, B3, FIP and BIP primers used in the study for LAMP assay. Point mutation highlighted by yellow (T958M → ACG-ATG) was inserted at 3’ end of FIP in mutation specific primers. (B) Schematic representation of designed LAMP primers F3, B3, FIP (F1c + F2) and BIP (B1c + B2) specific for amplifying wild types (T958) and for mutation (958 M). F1c and B1c sequences are complementary to F1 and B1.