Fig. 3.

The effects of brief exposure to HFD are transient on bone regeneration in Ldlr^−/− male mice. Line graphs of bone callus volume (mm³) for each group of mice fed either (A) low-fat diet (LFD) or (B) high-fat diet (HFD) over time. Bar graphs showing callus bone volume (BV) comparison between groups of male and female mice at (C,D) 14 dpr and (E,F) 21 dpr under (C,E) LFD or (D,F) HFD. Bar graphs showing callus BV comparison between (G-H) groups of male mice with deletion of the low-density lipoprotein (LDL) receptor (Ldlr^−/−) versus wild-type (WT) control and (I,J) female mice Ldlr^−/− versus WT under the same or different dietary condition (LFD or HFD). Data are displayed as the mean ± SD, considering WT male mice under LFD (n = 12), WT male mice under HFD (n = 11), Ldlr^−/− male mice under LFD (n = 10), Ldlr^−/− male mice under HFD (n = 11), WT female mice under LFD (n = 7), WT female mice under HFD (n = 8), Ldlr^−/− female mice under LFD (n = 6), and Ldlr^−/− female mice under HFD (n = 8). All BV measurements were obtained from live micro-computed tomography data analyzed using AnalyzePro 14.0 software. Statistical analysis was performed using two-way analysis of variance (ANOVA) followed by Fisher’s LSD test. Significant differences between groups are expressed as p < 0.05. dpr = days post-resection.