Fig. 2.

Differentially expressed proteins in the aqueous humor of patients with nAMD and control group. Quantitation of VEGFR1 and VEFGR2, which trigger the angiogenic action of VEGF, showed that they significantly upregulated in nAMD compared to control. The cutoff criteria for protein quantitation were as follows: t-test, p < 0.05, minimum confidence level > 95%, more than two unique peptides, FDR < 5%, and fold-change thresholds (expressed as log2 ratio) of > 1.5 or < − 1.5. For the analysis of differentially expressed proteins and statistical analysis, Perseus (version 1.5.8.2) was used, where the cutoff criteria for significant fold change (FC) and t-test p values were set at ± 1.5 and 0.05, respectively.