Fig. 5.

Inhibitory effects of apatinib on VEGFA (b) and αSMA (c) expression in the mouse model of laser-induced choroidal neovascularization (CNV). Intravitreal (IVT) injection of apatinib (1 µg in 1 µL DMSO) and subconjunctival (subconj) injection of apatinib (5 µg in 1 µL DMSO) were administered immediately after laser photocoagulation. Equal amounts of protein from eye tissue lysates of mice with laser-induced CNV were analyzed for the expression of the indicated proteins. Protein expression in the eyes of mice with laser-induced CNV was measured 1 week after laser photocoagulation by Western blot analysis (a). Data were analyzed using one-way ANOVA (*p < 0.05 and **p < 0.01 vs. non-laser exposed group, ^#p < 0.05 and ^##p < 0.01 vs. only laser exposed group). The results are expressed as mean ± S.E. (n = 5–12). Uncropped blot images are presented in supplementary information (Supplementary Fig. S4).