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. 2024 Sep 30;27(11):111085. doi: 10.1016/j.isci.2024.111085
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

InVivoMAb anti-mouse OX40 (CD134) Bio X cell Cat#BE0031
InVivoMAb anti-mouse PD-1 (CD279) Bio X cell Cat#BE0146
Purified Rat Anti-Mouse CD8a BD PharmingenTM Cat#553027; RRID: AB_394565
Biotin Rat Anti-Mouse Granzyme B Monoclonal Antibody eBioscience Cat#13-8822-82; RRID: AB_1603282
Mast Cell Tryptase (G-12) Santa Cruz Cat#Sc-32474; RRID: AB_2206484
Purified Rat Anti-Mouse IL-10 BD Pharmingen Cat#554464; RRID: AB_398557

Biological samples

Pancreatic disease patients samples UT MD Anderson Cancer Center www.mdanderson.org

Chemicals, peptides, and recombinant proteins

cromolyn Sigma Cat#C0399
masitinib Sigma Cat#SML3594
D-Luciferin Caliper Life Sciences Cat#122796
Toluidine blue VWR Cat#0672-25G

Critical commercial assays

RT2 SYBR@ Green Master Mixes SABiosciences Cat#330502
First Strand Kits SABiosciences Cat#330401
RT2 ProfilerTM PCR Array Mouse Allergy & Asthma SABiosciences Cat#PAMM-067ZD-12
RT2 ProfilerTM PCR Array Mouse Toll-Like Receptor SABiosciences Cat#PAMM-018ZD-12
Trichome-Masson Kit Sigma-Aldrich Cat#HT15

Experimental models: Cell lines

Panc02 mouse pancreatic cancer cell line https://doi.org/10.1158/0008-5472.CAN-12-4479 NA
KPC mouse pancreatic cancer cell line https://doi.org/10.1158/0008-5472.CAN-12-4479 NA

Experimental models: Organisms/strains

C57BL/6 wild type mice The Jackson Laboratory Cat#000664
C57BL/6 albino mice (B6(Cg)-Tyrc-2J/J) The Jackson Laboratory Cat#000058
mast cell-deficient mice (KitW-sh/W-sh) The Jackson Laboratory Cat#012861
IL-10–deficient mice (B6.129P2-IL10tmiCgn/J) The Jackson Laboratory Cat#002251
PGE2-deficient mice (B6.129[FVB]-Ptgs2tm2.1(ptgs1)Fn/J) The Jackson Laboratory Cat#008104
cLGL-KRasG12V/Ela-CreER mice https://doi.org/10.1053/j.gastro.2009.05.052 NA
KPC-GEMM (KrasG12D/+; Trp53R172H/+; Pdx-1-Cre) Frederick National Laboratory for Cancer Research (NCI, Maryland, USA
https://doi.org/10.1016/j.ccr.2005.04.023
NA
KC-GEMM (KrasG12D/+; Pdx-1-Cre) Frederick National Laboratory for Cancer Research (NCI, Maryland, USA
https://doi.org/10.1016/S1535-6108(03)00309-X
NA

Software and algorithms

ImageJ https://wsr.imagej.net/notes.html Version 1.52p 22 June 2019
Living Image IVIS Spectrum in https://store.caliperls.com 3.1 Software
TCGA cBioPortal http://www.cbioportal.org/index.do NA
Prism https://www.graphpad.com NA
RT2 Profiler PCR Array Data Analysis http://pcrdataanalysis.sabiosciences.com NA
scTIME Portal http://sctime.sklehabc.com NA
Single cell RNA sequencing data http://sctime.sklehabc.com/#/visual/11/CRA001160?species=%5B%22Homo%20sapiens%22%5D&addpath&compare=trueA NA