REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
InVivoMAb anti-mouse OX40 (CD134) | Bio X cell | Cat#BE0031 |
InVivoMAb anti-mouse PD-1 (CD279) | Bio X cell | Cat#BE0146 |
Purified Rat Anti-Mouse CD8a | BD PharmingenTM | Cat#553027; RRID: AB_394565 |
Biotin Rat Anti-Mouse Granzyme B Monoclonal Antibody | eBioscience | Cat#13-8822-82; RRID: AB_1603282 |
Mast Cell Tryptase (G-12) | Santa Cruz | Cat#Sc-32474; RRID: AB_2206484 |
Purified Rat Anti-Mouse IL-10 | BD Pharmingen | Cat#554464; RRID: AB_398557 |
Biological samples | ||
Pancreatic disease patients samples | UT MD Anderson Cancer Center | www.mdanderson.org |
Chemicals, peptides, and recombinant proteins | ||
cromolyn | Sigma | Cat#C0399 |
masitinib | Sigma | Cat#SML3594 |
D-Luciferin | Caliper Life Sciences | Cat#122796 |
Toluidine blue | VWR | Cat#0672-25G |
Critical commercial assays | ||
RT2 SYBR@ Green Master Mixes | SABiosciences | Cat#330502 |
First Strand Kits | SABiosciences | Cat#330401 |
RT2 ProfilerTM PCR Array Mouse Allergy & Asthma | SABiosciences | Cat#PAMM-067ZD-12 |
RT2 ProfilerTM PCR Array Mouse Toll-Like Receptor | SABiosciences | Cat#PAMM-018ZD-12 |
Trichome-Masson Kit | Sigma-Aldrich | Cat#HT15 |
Experimental models: Cell lines | ||
Panc02 mouse pancreatic cancer cell line | https://doi.org/10.1158/0008-5472.CAN-12-4479 | NA |
KPC mouse pancreatic cancer cell line | https://doi.org/10.1158/0008-5472.CAN-12-4479 | NA |
Experimental models: Organisms/strains | ||
C57BL/6 wild type mice | The Jackson Laboratory | Cat#000664 |
C57BL/6 albino mice (B6(Cg)-Tyrc-2J/J) | The Jackson Laboratory | Cat#000058 |
mast cell-deficient mice (KitW-sh/W-sh) | The Jackson Laboratory | Cat#012861 |
IL-10–deficient mice (B6.129P2-IL10tmiCgn/J) | The Jackson Laboratory | Cat#002251 |
PGE2-deficient mice (B6.129[FVB]-Ptgs2tm2.1(ptgs1)Fn/J) | The Jackson Laboratory | Cat#008104 |
cLGL-KRasG12V/Ela-CreER mice | https://doi.org/10.1053/j.gastro.2009.05.052 | NA |
KPC-GEMM (KrasG12D/+; Trp53R172H/+; Pdx-1-Cre) | Frederick National Laboratory for Cancer Research (NCI, Maryland, USA https://doi.org/10.1016/j.ccr.2005.04.023 |
NA |
KC-GEMM (KrasG12D/+; Pdx-1-Cre) | Frederick National Laboratory for Cancer Research (NCI, Maryland, USA https://doi.org/10.1016/S1535-6108(03)00309-X |
NA |
Software and algorithms | ||
ImageJ | https://wsr.imagej.net/notes.html | Version 1.52p 22 June 2019 |
Living Image | IVIS Spectrum in https://store.caliperls.com | 3.1 Software |
TCGA cBioPortal | http://www.cbioportal.org/index.do | NA |
Prism | https://www.graphpad.com | NA |
RT2 Profiler PCR Array Data Analysis | http://pcrdataanalysis.sabiosciences.com | NA |
scTIME Portal | http://sctime.sklehabc.com | NA |
Single cell RNA sequencing data | http://sctime.sklehabc.com/#/visual/11/CRA001160?species=%5B%22Homo%20sapiens%22%5D&addpath&compare=trueA | NA |