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E2 SP is required for RLP assembly. CHO-E2E1 cells were transfected with expression vectors encoding different capsid protein constructs, and at 48 h posttransfection, cells were prepared for routine morphologic examination by embedding them in Epon. (A) Electron micrograph of CHO-E2E1 cells transfected with CapE2SP. RLPs (arrowheads) can be seen in the Golgi complex (G) of these cells. No RLPs were observed in the Golgi complex of CapCD8SP-transfected cells (B). Bar, 100 μm.
