REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
| ||
Antibodies | ||
| ||
Pneumococcal serotype multiplex in-house assay (PCV13-MIA) | RIVM, the Netherlands, Elberse et al., 2010 | IIV-IMS-MO22-MIA Pneumo |
Biotinylated Anti-Human IgG (gamma) Antibody | KPL, Gaithersberg Maryland | Cat# 16-10-02; RRID:AB_2732814 |
Biotinylated Anti-Human IgA (alpha chain) Antibody | KPL, Gaithersberg Maryland | Cat# 16–10-01; RRID:AB_2732815 |
| ||
Bacterial and Virus Strains | ||
| ||
Rotavirus antigen, supernatant from clarified Rotarix extract (~106 FFU/ml), Rotarix-infected MA104 cell cultures are clarified by centrifugation. | CDC, Atlanta, GA | N/A |
| ||
Biological Samples | ||
| ||
Rabbit hyperimmune serum to Rhesus rotavirus | CDC, Atlanta, GA | CD 08, 10–5-01 |
| ||
Chemicals, Peptides, and Recombinant Proteins | ||
| ||
Extravidin | Sigma Aldrich, St Louis, MO (Sigma-Aldrich, RRID:SCR_008988 | Cat# E2886, ExtrAvidin FITC, Sigma-Aldrich; RRID:SCR_013728 |
| ||
Critical Commercial Assays | ||
| ||
ProspecT Rotavirus microassay | Thermo Fischer Scientific, Oxoid, UK | Cat# R240396; RRID:AB_2732820 |
anti-Tetanus Toxoid IgG Enzyme Immunoassay Kit | The Binding Site, Birmingham, England (Binding Site, RRID:SCR_004051) | Cat# MK010; RRID:AB_2732816 |
anti-PCP IgG Enzyme Immunoassay Kit | The Binding Site, Birmingham, England (Binding Site, RRID:SCR_004051) | Cat# MK012; RRID:AB_2732817 |
| ||
Deposited Data | ||
| ||
Sequence data | This paper | ENA Accession Number PRJEB25634 |
Full analysis workflows for microbiome dada2 ASV resolution, statistical analysis and plotting | This paper | https://github.com/shandley/human_volunteer_rotabiome |
| ||
Software and Algorithms | ||
| ||
Prism7 software | Graphpad, San Diego, CA | Version 7.0c; RRID:SCR_002798 |
RStudio | RStudio, Inc | Version 1.0.143; RRID:SCR_000432 |
PhyloSeq | McMurdie and Holmes, 2013 | https://joey711.github.io/phyloseq/; RRID:SCR_013080 |
Dada2 | Callahan et al., 2016 |
https://github.com/benjjneb/dada2; RRID:SCR_008205 |