MORC3 Is a Target of the Influenza A Viral Protein NS1

. Author manuscript; available in PMC: 2024 Oct 28.

Published in final edited form as: Structure. 2019 Apr 18;27(6):1029–1033.e3. doi: 10.1016/j.str.2019.03.015

KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
Escherichia coli BL21 (DE3) RIL	Andrews et al., 2016	N/A
Escherichia coli Rosetta-2 (DE3) pLysS	Ali et al., 2012	N/A
Chemicals, Peptides, and Recombinant Proteins
Dithiothreitol	Gold Biotechnology	27565-41-9
NS1(222-230), NS1K229me3(222-230), H3(1-12) and H3K4me3(1-12)	Synpeptide	N/A
¹⁵NH₄Cl	Sigma-Aldrich	299251
IPTG	Gold biotechnology	I2481C100
Ni-NTA beads	Thermo Fisher Sci	88223
Glutathione Sepharose 4B beads	Thermo Fisher Sci	16101
PreScission and Ulp1 proteases	Home expressed	N/A
Deposited Data
MORC3-CW:NS1 complex	This study	PDB: 6O5W
MORC3-CW:H3K4me3 complex	Andrews et al., 2016	PDB: 5SVX
Experimental Models: Cell Lines
Human: HEK293T	ATCC	CRL-11268
Recombinant DNA
Plasmid: modified pGEX6p-1	Andrews et al., 2016	N/A
Software and Algorithms
Phenix	Adams et al., 2010	N/A
Coot	Emsley et al., 2010	N/A
MOLProbity	Chen et al., 2010	N/A
Genevestigator		https://www.genevestigator.ethz.ch/