Figure 1.

Cryo-EM structures of ex vivo New1-ribosome complexes. (A–F) cryo-EM maps of New1p (blue) in complex with (A–E) elongating (states 1–6) and (F) termination (State 7) state ribosomes. The 40S and 60S subunits are colored yellow and grey, respectively and the uL1 protein (pink) of the L1 stalk is indicated in the ‘in’, ‘out’ or intermediate (‘inter’) conformations. A-tRNA (red), P-tRNA (orange), E-tRNA (cyan), A/P-tRNA (dark blue), P/E-tRNA (green), P/E*-tRNA (dark blue), eIF-5A (lime) and eRF1 (magenta) are colored. The schematics below the maps indicate the conformation of the tRNAs in the (A–C) pre-translocational states (states 1–3) and (D–F) post-translocational states (states 4–7). As the ribosomes are shown in the same pose on panels A–F, the pictogram view provided on panel A is applicable to all of the panels.