Figure 2.

Depletion of CcrM results in slow growing, elongated and swollen cells when cultivated in complex media. (A) JC2141 (WT’) and JC2307 (ΔccrM Ptac-ccrM) cells were plated onto ATGN, YEB or LB agarose plates containing or not the IPTG inducer and colonies were then cultivated for ∼3 days prior to imaging. (B) JC2141 and JC2307 strains were pre-cultured into liquid ATGN, YEB or LB media + IPTG. Cells from over-night cultures were then washed and diluted to an OD₆₀₀∼0.1 into the indicated medium and placed into a plate reader to record the OD₆₀₀ every 15 min. Error bars represent means ± standard deviations from three biologically independent replicates. Note that the two WT’ curves overlap for the LB growth condition. (C) JC2141 and JC2307 strains were pre-cultured into liquid ATGN + IPTG and then diluted into ATGN or YEB or LB with IPTG. Once cultures reached exponential phase again, cultures were washed and cells were resuspended to an OD₆₀₀∼0.2 into ATGN, YEB or LB ± IPTG at time T0. Cells were then fixed and imaged by phase-contrast microscopy after 24 h of growth. White scale bars inside images are 5 μm.