Table 1.
Oligonucleotide primer sequences used for the RT-qPCR analysis.
| Gene | Primer sequence (5’-3’) | Product length (bp) | Accession number |
|---|---|---|---|
| Bax | F: ATCCAAGACCAGGGTGGCTG | 148 | NM_007527.3 |
| R: CAGTCCAAGGCAGTGGGAG | |||
| H2ax | F: GTGCTGCTGCCCAAGAAGA | 138 | NM_010436.2 |
| R: CCTTTGTGGAGGTGTGGGGA | |||
| Pten | F: ACTTTGAGTTCCCTCAGCCA | 93 | NM_008960.2 |
| R: ACATTTTGTCCTTTTTGAGCATCT | |||
| Tap63 (Trp63) | F: CTGCTCATCATGCCTGGACT | 124 | NM_001127264.1 |
| R: CCAGATGGCATGTCGGAACT | |||
| Ybx2 | F: CCCAACAGCCTACCACAGAA | 173 | NM_016875.3 |
| R: GATAGGCGCGGAGGTCTCT | |||
| Noxa (Pmaip1) | F: GAGTGCACCGGACATAACTG | 107 | NM_021451.2 |
| R: CGTCCTTCAAGTCTGCTGG | |||
| Puma (Bbc3) | F: TTGAACAGTGAACACTCTTTTTGT | 70 | NM_133234.2 |
| R: CATGTCCTTACAGGTAGTGCC | |||
| Casp 3 | F: GAGCTTGGAACGGTACGCTAA | 114 | NM_001284409.1 |
| R: CCACTGACTTGCTCCCATGT | |||
| Casp 6 | F: GCTCAAAATTCACGAGGTG | 110 | NM_009811.4 |
| R: TTTTGGCGTCGTATGCGTAA | |||
| Casp 8 | F: ATCGGTAGCAAACCTCTGTGA | 169 | NM_009812.2 |
| R: GGTAGAAGAGCTGTAACCTGTG | |||
| Pdk1 | F: GATCCTGTCACCAGCCAAAA | 104 | NM_001360002.1 |
| R: CCACCGAACAATAAGGAGTGC | |||
| Pcna | F: TTGCACGTATATGCCGAGACC | 104 | NM_011045.2 |
| R: CCGCCTCCTCTTCTTTATCCA | |||
| Mki67 | F: TTTAACTCAGCGCCTTGTTCC | 167 | NM_001081117.2 |
| R: GCATTCCCTCACTCTTGTTAATG | |||
| P21 (Cdkn1a) | F: ATCCAGACATTCAGAGCCACA | 181 | NM_007669.5 |
| R: AGTCAAAGTTCCACCGTTCTC | |||
| Sod1 | F: GCGGATGAAGAGAGGCATGTT | 121 | NM_011434.2 |
| R: GGCCAATGATGGAATGCTCT | |||
| Trp53 | F: CCGAAGACTGGATGACTGC | 167 | NM_001127233.1 |
| R: CTCCTCAACATCCTGGGGC | |||
| Bcl-2 | F: TCTTTGAGTTCGGTGGGGTC | 154 | NM_009741.5 |
| R: AGTTCCACAAAGGCATCCCAG | |||
| Foxo3a | F: CTCATGGATGCTGACGGGTT | 174 | NM_001376967.1 |
| R: GTTTGAGGGTCTGCTTTGCC | |||
| Mtor | F: GTTGGCATAACAGATCCTGACCC | 125 | NM_020009.2 |
| R: TGGTCATTCAGAGCCACAAAC | |||
| Amh | F: TTGCTGAAGTTCCAAGAGCC | 137 | NM_007445.3 |
| R: GTAGGGCAGAGGTTCTGTGTG | |||
| Tgfb1 | F: AACTATTGCTTCAGCTCCACAG | 159 | NM_011577.2 |
| R: CTGTGTGTCCAGGCTCCAAA | |||
| Smad2 | F: TTGCCTCCAGTCTTAGTGCC | 149 | NM_001252481.1 |
| R: CCAGGTGGTGGTGTTTCTGG | |||
| Smad3 | F: TCAGAGAGTAGAGACGCCAGTT | 167 | NM_016769.4 |
| R: CAGGAGGTGGGGTTTCTGGAATA | |||
| Stat3 | F: GGAAGCGAGTGCAGGATCTA | 97 | NM_213659.3 |
| R: TCTCCTTGGCTCTTGAGGGT | |||
| Yap1 | F: TCCAACCAGCAGCAGCAAAT | (41) | NM_001171147 |
| R: TTCCGTATTGCCTGCCTGCCGAAA | |||
| Mst1 | F: CAGCCCGAGGAAGTGTTTGA R: CACGGGCACTTGCTTGATTG |
(41) | XM_006499963.1 |
| Lats2 | F: CTTTGCTTCCCTCGGACAGT | (41) | NM_010690.1 |
| R: AACTGGGTGTAGTCAGGGGT | |||
| Kitl | F: TCAACTATGTCGCCGGGATG R: CGAAATGAGAGCCGGCAATG |
(42) | (42) |
| Cd31 | F: CCAAAGCCAGTAGCATCATGGTC R: GGATGGTGAAGTTGGCTACAGG |
(43) | NM_008816 |
| β-actin (Actb) | F: TCCTTCTTGGGTATGGAATCCT | 81 | NM_007393.5 |
| R: TTTACGGATGTCAACGTCACAC |