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. Author manuscript; available in PMC: 2024 Oct 28.
Published in final edited form as: Nature. 2024 Aug 14;634(8032):96–103. doi: 10.1038/s41586-024-07830-1

Extended Data Fig. 10: Functional analysis of lungfishes ZRS and SAG treatment of Lepidosiren paradoxa regenerating fins.

Extended Data Fig. 10:

a, Mouse transgenesis and LacZ staining for the Neoceratodus forsteri and Lepidosiren paradoxa ZRS sequences. Genotyping indicates whether insertion was either in a single or double copy at the targeted locus, or randomly integrated in the genome. Neoceratodus forsteri ZRS gives ZPA staining in 16/16 embryos, whereas the Lepidosiren paradoxa ZRS does not give staining in 15/15 embryos. b, Regeneration of pectoral fins in presence of the shh agonist SAG does not result in radial growth in Lepidosiren paradoxa (n = 3 for SAG treated animals, n = 3 for DMSO-treated animals; representative images of one animal per treatment are shown).