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. 2024 Oct 28;30:190. doi: 10.1186/s10020-024-00946-0

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — Impact of MECOM Regulation of Smad Proteins on Apoptosis and Cell Cycle Regulation in HEC-1-A Cells. A Western blot analysis of changes in Smad protein levels after MECOM knockout and overexpression. The left image shows the changes in Smad protein levels in HEC-1-A cells after MECOM knockout and overexpression. The right chart shows the quantitative analysis (Compared to the Control group, ****p < 0.0001; ns: insignificant). B Western blot analysis of the impact of MECOM knockout on apoptosis-related proteins. The left image shows the protein expression levels of BAX, caspase-3 (CASP3), Bcl-2, and Bcl-xL in the control group (Control), empty vector control group (V2-NC), and MECOM knockout (V2-MECOM). The right chart is the quantitative analysis of the corresponding proteins, showing a significant increase in the expression of Smad2/3, BAX, and caspase-3 in cells with MECOM knockout, while the expression of Bcl-2, Bcl-xL significantly reduced (Compared to the Control group, ****p < 0.0001; ns: not significant). C Western blot analysis of the impact of MECOM knockout on cell cycle regulatory proteins. The left image shows the protein expression levels of CHK1, CHK2, p21, and CDK2 in the control group (Control), empty vector control group (V2-NC), and MECOM knockout (V2-MECOM). The right chart is the quantitative analysis of the corresponding proteins, showing a significant increase in the expression of Smad2/3, CHK1, CHK2, and p21 in cells with MECOM knockout, while the expression of CDK2 significantly reduced (Compared to the Control group, ****p < 0.0001; ns: not significant). All cell experiments were repeated three times