Table 1.
The primer sequence of qRT-RCR
| Target | Sequence (5'-3') |
|---|---|
| MECOM | F: AATATGAGTCATGCCAACCC |
| R: CTTGGTGTACTGACATCATC | |
| GAPDH | F: TGCACCACCAACTGCTTA |
| R: GGATGCAGGGATGATGTTC | |
| PRDM1 | F: AAGCAACTGGATGCGCTATGT |
| R: GGGATGGGCTTAATGGTGTAGAA | |
| PRDM2 | F: AAGTGAGCCGAGTTTCACCTC |
| R: CTAATCGCTCGTCTGGTTCTG | |
| PRDM4 | F: TCCTCTGTGAGCAATGCCTTG |
| R: CCACACATCACCCCTCGAT | |
| PRDM5 | F: TACGTGCCGGACAGGTTCT |
| R: TTCACCCTTTCGCACTCTGC | |
| PRDM6 | F: GGTGGGGAACCTAGTAAGTCG |
| R: ACCGTTGAAGGGACATTTAAGTT | |
| PRDM7 | F: GCAGGGAGAGAACCAAAGCC |
| R: ACTTATCACTGAAACCTTGCCC | |
| PRDM8 | F: TTTTACCAGCGTTTACACCACC |
| R: GCTGTCATATAGGGAAGTATGGC | |
| PRDM9 | F: CAGCCAACAATGGATACTCCTG |
| R: CTGGCCGTATTCATCCCCA | |
| PRDM10 | F: GCTGCCTTCCATCGAGAGTG |
| R: CCAGTCATCCAGATCCGTGTC | |
| PRDM11 | F: GTCTGCTCACCACTCCGAG |
| R: TTGGGCATTCATCCACGAAGT | |
| PRDM12 | F: CAAGGCGGGAACCGAGATG |
| R: CACATGAGGTTGTTGTTCTTGC | |
| PRDM13 | F: TGGAGTGGATAGGGTTAATCCG |
| R: CGGGTAAGTCTGCAATAGCTTC | |
| PRDM14 | F: ACACGCCTTTCCCGTCCTA |
| R: GGGCAGATCGTAGAGAGGCT | |
| PRDM15 | F: ACTTGGAGATCAGACGACTGG |
| R: TGGACTCAAAGGGACCGAACT | |
| PRDM16 | F: CGAGGCCCCTGTCTACATTC |
| R: GCTCCCATCCGAAGTCTGTC |