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Anterograde directional flow analysis by brain histology after in vivo nanoparticle administration into the cisterna magna. (a) Gold Enhancement showed staining of lymphatic vessels in the cervical region (red arrows) (n=3). (b) Chinese ink nanoparticles were identified in the cervical lymphatic vessels (red arrows), the subarachnoid space (black arrows), as well as the cervical spinal cord (yellow arrow), peripheral nerves (Orange arrow), and connective tissue (blue arrows) (n=3).
