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. 2001 Feb;75(4):2002–2009. doi: 10.1128/JVI.75.4.2002-2009.2001

FIG. 3.

FIG. 3

RT-PCR analysis of total RNA isolated from heart muscle for an adult B. umbellus animal (BOUM) and 8-day-old embryos of P. colchicus (PHCO) and C. virginianus (COVI). (A) RT-PCR on total RNA. Lane 1, 100-bp ladder (Promega); lanes 2 to 5, primers GAG.F1 and GAG.R1; lanes 6 to 9, primers GC2128F and GC2604R. (B) RT-PCR using β-actin primers (lanes 2 to 5) and PCR using primers GAG.F1 and GAG.R1 on DNase-treated RNA extracts (lanes 6 to 9). (C) PCR on non-DNase-treated samples using primers GAG.F1 and GAG.R1.