FIG. 5.

Southern blot analysis of HindIII-digested avian genomic DNA using TERV probe^matrix and probe^env. Hybridization was performed at 65°C, and the filter was washed with salt-SDS solution at concentrations as low as 0.5× SSPE–0.5% SDS at 65°C. (A) Probe^matrix. (B) Probe^env. Lanes 1 and 2, B. umbellus (ruffed grouse); lanes 3, Bonasa bonasia (hazel grouse); lanes 4, Lagopus lagopus (willow ptarmigan); lanes 5, Lagopus leucurus (white-tailed ptarmigan); lanes 6, Dendragapus falcipennis (Siberian grouse); lanes 7, Anseriformes (Aythya americana [redhead]); lanes 8, G. gallus (domestic chicken). This shows the presence of TERV or closely related elements in all tetraonine birds surveyed.