Fig. 1.

Summary of developmental zebrafish toxicity assay (ZF Dev. Tox.), Ahr2 dependence in developmental zebrafish toxicity assay (AHR2 Dep. DZF), Cyp1a induction (ZF Cyp1a IHC), and AHR activation in the AHR reporter assay for zebrafish Ahr2 (ZF Ahr2) and human AHR. Positive Ahr2 dependence indicates attenuated toxicity in Ahr2-null fish. Positive Cyp1a induction indicates detection of Cyp1a via IHC after exposure. Positive results in either reporter assay indicates robust expression of luciferase sufficient to estimate an EC₅₀ with exposures up to 30 µM using a 4 parameter log-logistic model. Asterisk (*) indicates some significant activation at the highest concentrations, but for which a log-logistic model was not significant. Significance of highest concentrations in these cases were determined by fold changes from controls greater than 2 and 95% confidence intervals not including 1. Dagger (‡) indicates Ahr2 dependence was determined by morpholino knockdown.