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. 2024 Oct 28;24:419. doi: 10.1186/s12883-024-03924-5

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 12 — The expression of EMT-related proteins in LN229 cells and U87-MG cells after overexpression of TGFBI and pretreatment with FAK inhibitors. (A-B) Western blot was used to detect the expression levels of N-cadherin and Vimentin in LN229 cells and U87-MG cells. (C-D) immunofluorescence analysis showed the expression levels of N-cadherin and Vimentin in LN229 cells and U87-MG cells. The magnification of the image is 200×. Vector: transfect empty plasmid. OE-TGFBI: transfect TGFBI overexpression plasmid. Vector + inhibitor: transfect empty plasmid and pretreatment with PF-573,228 for 1 h before the experiment. OE-TGFBI + inhibitor: transfect TGFBI overexpression plasmid and pretreatment with PF-573,228 for 1 h before the experiment