Table 1. Bas2p dependence of Bas1p–VP16 recruitment to different ADE genes.

	– ADE			+ ADE
Fusion	+ Bas2pa	– Bas2pb	Ratioc	+ Bas2pa	– Bas2pb	Ratiod
HIS4–LacZ	412	44	0.11	187	31	0.16
ADE1–LacZ	535	379	0.71	389	379	0.97
ADE2–LacZ	99	70	0.71	80	75	0.94
ADE4–LacZ	438	406	0.93	390	371	0.95
ADE5,7–LacZ	176	62	0.35	97	71	0.73
ADE8–LacZ	112	101	0.90	99	98	0.99
ADE13–LacZ	175	138	0.79	113	110	0.97
ADE17–LacZ	652	513	0.78	520	423	0.81

The yeast strain Y329 (gcn4bas1BAS2) or L4233 (gcn4bas1bas2) was transformed with a plasmid carrying one of the LacZ fusions listed and either the B836 empty vector or the plasmid carrying the BAS1–VP16 gene. Six to 12 clones of each transformation were grown overnight in SC medium lacking uracil and leucine and then diluted to 0.1 OD₆₀₀ in the same medium supplemented (+ ADE) or not (– ADE) with adenine. After 6 h incubation at 30°C the β-galactosidase (β-Gal) assays were performed as described in Materials and Methods. Results given in the table correspond to β-Gal units obtained with the BAS1–VP16 plasmid subtracted from those obtained with the control plasmid (B836).

^aβ-Galactosidase units measured in Y329 strain.

^bβ-Galactosidase units measured in L4233 strain.

^cRatio –Bas2p/+Bas2p in the absence of adenine.

^dRatio –Bas2p/+Bas2p in the presence of adenine