Fig. 4.

Sestrin2 was downregulated in vivo and in vitro and regulated by SETDB1. A The heatmap of top 10 targeted genes of SETDB1 (Txndc11, Sesn2, Tmem125, Sfi1, Eif4enif1, Nefm, Gm525, Sspn, Plagl1, Cd209a). B The motif density of SETDB1 relative to the TSS. C The forward and reverse motif logo of SETDB1. D qPCR detection of SESN2 mRNA levels in mice kidney tissues. E WB detection of Sestrin2 protein levels in mice kidney tissues. F Representative images of immunohistochemistry of Sestrin2 in mice kidney tissues (left) and related quantitative analysis (right). Bar = 50 μm. G qPCR detection of SESN2 mRNA levels in HK-2 cells. H WB detection of Sestrin2 protein levels in HK-2 cells. I Representative images of immunofluorescence (red) of Sestrin2 in HK-2 cells (left) and related quantitative analysis (right). Bar = 50 μm. Values are expressed as the mean ± SEM. N = 3. The ** represents differences between groups, and p < 0.01