Fig. 5.

SETDB1 regulates H3K9me3 on the SESN2 promoter. A ChIP detection of the enrichment of SETDB1, H3K9me1, H3K9me2, H3K9me3, and H3K9ac on SESN2 promoter in vivo. B ChIP detection of SETDB1, H3K9me3, and H3K9ac on SESN2 promoter in vitro. C Detection of luciferase activity by the SESN2 promoter reporter gene in HK-2 cells. D ChIP detection of the enrichment of Flag and H3K9me3 on SESN2 promoter in HK-2 cells. E Schematic diagram of SESN2 promoters with different lengths. F ChIP detection of the enrichment of SETDB1 on SESN2 promoters with different lengths in HK-2 cells. G Detection of luciferase activity by the reporter gene of SESN2 promoters with different lengths in HK-2 cells. Values are expressed as the mean ± SEM. N = 3. The ** represents differences between groups, and p < 0.01