Fig. 6.

Knockdown of SESN2 eliminated mitochondrial protection mediated by (R,R)-59 in vivo and in vitro. A Representative images of immunohistochemistry of DRP1 and FIS1 in mice kidney tissues (left) and related quantitative analysis (right). Bar = 50 μm. B qPCR detection of FIS1, DRP1, and MFN2 mRNA levels. C WB detection of FIS1, DRP1, and MFN2 protein levels. D Representative images of Mito Tracker Red CMXRos in HK-2 cells (left) and related quantitative analysis (right). Bar = 30 μm. E Detection of JC-1 in HK-2 cells by flow cytometry. Values are expressed as the mean ± SEM. N = 3. The ** represents differences between groups, and p < 0.01