Fig. 8.

HP1β interacted with SETDB1 to jointly regulate SESN2. A CoIP results of SETDB1 and different HP1. B WB detection of HP1α and HP1β protein levels. C ChIP detection of the enrichment of HP1α and HP1β on SESN2 promoter in vivo. D Re-ChIP detection of the enrichment of SETDB1 combined with HP1β on SESN2 promoter in vivo. E WB detection of HP1β and Sestrin2 protein levels. F Detection of ROS levels in HK-2 cells by flow cytometry. G Representative images of Mito Tracker Red CMXRos in HK-2 cells (left) and related quantitative analysis (right). Bar = 30 μm. Values are expressed as the mean ± SEM. N = 3. The ** represents differences between groups, and p < 0.01