Table 1.
Descriptive statistics of blood cell ratios, ELISA levels (ELISA units), and serum electrophoretic fractions (g/l) for the enrolled dogs
|
Li− Median (Min–Max) [25th–75th] (n = 10) |
Li+ Median (Min–Max) [25th–75th] (n = 185) |
Li+healthy Median (Min–Max) [25th–75th] (n = 100) |
Li+sick Median (Min–Max) [25th–75th] (n = 85) |
Li+IIa/IIb Median (Min–Max) [25th–75th] (n = 66) |
Li+III/IV Median (Min–Max) [25th–75th] (n = 19) |
Mann–Whitney U-test P |
|
|---|---|---|---|---|---|---|---|
| NLR |
1.49 (1.05–3.91) [1.28–1.73]A,B,C,E,F |
3.1 (1–48) [2.3–4.5]A |
2.8 (1.3–30) [2.1–3.8]B,D,G,H |
3.7 (1–48) [2.6–5.7]C,D |
3.7 (1–48) [2.52–5.78]E,G |
4 (1.6–14.3) [3.3–5.55]F,H |
< 0.001A,B,C,D,E,F,G 0.002H |
| MLR |
0.18 (0.12–0.35) [0.15–0.22]C,F |
0.2 (0.0–1.3) [0.1–0.4] |
0.2 (0.1–1.3) [0.1–0.3]D,G,H |
0.3 (0–1.2) [0.2–0.4]C,D |
0.3 (0–1.2) [0.2–0.4]G |
0.3 (0.1–1.1) [0.3–0.5]F,H |
0.001G < 0.001D,H 0.005 F 0.034C |
| PLR |
123 (60.4–217) [94.1–160] |
123 (17.2–1290) [77.3–182] |
107 (40.5–267) [73.5–158]D, G |
150 (17.2–1290) [87.9–197]D |
163 (31.8–1290) [101–197]G |
136 (17.2–484) [82.4–193] |
0.012G 0.023D |
| ELISA |
5.79 (4.42–7.65) [4.97–6.24] |
241 (4–11,114) [119–813] |
142 (4–1210) [101–250]D,G,H |
752 (4.4–11,114) [197–2933]D |
616 (87.2–8594) [187–2136]G |
1794 (4.4–11,114) [281–3405]H |
< 0.001D,G,H |
| Albumin |
32.5 (29.7–35.7) [31.8–33.3]B,F |
34.6 (15.3–49.1) [31.1–37.8] |
36.5 (25.5–45.4) [34.3–39]B,D,G,H |
31.6 (15.3–49.1) [27.1–34.6]D |
32.9 (21.2–49.1) [28.8–35.3]G,I |
27.3 (15.3–34.1) [22.6–30.7]F,H,I |
< 0.001B,D,F,G,H,I |
| α1-Globulin |
3.45 (3–4) [3.18–3.65] |
3.5 (1.7–10.2) |
3.4 (1.7–4.8) [2.9–3.73]D,G,H |
3.7 (2–10.2) [3.2–4.3]D |
3.7 (2–5.9) [3.2–4.27]G |
3.7 (2.8–10.2) [3.25–4.6]H |
0.008G 0.002D 0.013H |
| α2-globulin |
6.9 (5.3–8.6) [5.95–7.38]C,E,F |
7.2 (2.8–18.1) [6.2–8.7] |
6.5 (2.8–12.7) [5.7–7.5]D,G,H |
8.4 (4.5–18.1) [7.1–9.5]C,D |
8.45 (4.9–18.1) [7.23–9.17]E,G |
8.1 (4.5–13.6) [6.85–11]F,H |
< 0.001D,G,H 0.004C,E 0.020 F |
| β-globulin |
7.1 (6.5–8.3) [6.95–7.73]A,B,C,E,F |
12.9 (1.4–37.9) [11.4–15.4]A |
12.1 (1.4–24.3) [10.4–14.2]B,D,G,H |
14.1 (9.2–37.9) [12.1–17.7]C,D |
13.9 (9.2–37.9) [12.1–16.9]E,G |
15.3 (10.3–24.8) [13.1–19.9]F,H |
< 0.001A,B,C,D,E,F,G,H |
| γ-globulin |
3.45 (2.6–4.7) [3.2–4.17]A,B,C,E,F |
9.8 (4.4–56) [7.7–14.1]A |
8.2 (4.4–13.7) [7–9.4]B,D,G,H |
14.5 (5.3–56) [11–21.6]C,D |
14.1 (5.3–50.8) [11–19]E,G,I |
22.1 (8.6–56) [12.3–30]F,H,I |
< 0.001A,B,C,D,E,F,G,H 0.024I |
Min minimum, Max maximum, 25th 25th percentile, 75th 75th percentile, P P-values, NLR neutrophil-to-lymphocyte ratio, MLR monocyte-to-lymphocyte ratio, PLR platelet-to-lymphocyte ratio, Li− anti-L. infantum antibody-negative healthy dogs, Li+ anti-L. infantum antibody-positive dogs, Li+healthy Li-seropositive healthy dogs, Li+sick dogs with clinical and/or clinicopathological abnormalities, Li+IIa/IIb dogs in LeishVet stage IIa/IIb, Li+III/IV dogs in LeishVet stage III/IV. § = PLR was calculated in 100 dogs, 7 Li−, 54 Li+healthy, 39 Li+sick, 27 Li+IIa/IIb dogs, and 12 Li+III/IV dogs respectively. Endpoint ELISA results were evaluated for each group of Li+ dogs. Significant comparisons: A = Li+ > Li−; B = Li+healthy > Li−; C = Li+sick > Li−; D = Li+sick > Li+healthy; E = Li+IIa/IIb > Li−; F = Li+III/IV > Li−; G = Li+IIa/IIb > Li+healthy; H = Li+III/IV > Li+healthy; I = Li+III/IV > Li+IIa/IIb