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. 2000 Dec 1;28(23):4623–4633. doi: 10.1093/nar/28.23.4623

Figure 5.

Figure 5

3′-End determination of the small separate 5′ 23S rRNA domain from sequence analysis of RNA linker ligation-mediated cDNA cloning using total RNA from Bradyrhizobium USDA 4362 and USDA 4377 or gel-purified low molecular weight RNA from LSU ribosomes of R.palustris. (a) For R.palustris, a larger sample of clones was examined than in the initial analysis (20) though the same highly purified RNA preparation was used as template. (b) Total RNA from USDA 4377 and (c) USDA 4362 was used as template. Two major clusters of RNA 3′ endpoints are detected in each of these cases corresponding to incompletely and completely processed rRNA. The incompletely processed RNA 3′-ends regions lie close to the RNase III cleavage sites on the left side of stem–loop structure #1 mapped previously by primer extension using in vitro processed R.palustris precursors (20). Putative mature 3′-ends from USDA 4377 and USDA 4362 RNAs correspond closely to those from RNA isolated directly from R.palustris LSU ribosomes (A).