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. 2005 Jun 16;33(11):3492–3502. doi: 10.1093/nar/gki659

Figure 4.

Figure 4

Sequence-specificity of the TFO-induced recombination. (A) Triplex-induced recombination with a pyrimidine motif target. Cells pre-transfected with the dual supF plasmid containing the 18 bp A–T-rich site, pSupFTC18, were transfected the next day with lipid reagent alone or with the indicated TFOs. AG30 and SCR30 are G-rich TFOs and MIX30 has a mixed sequence. Shuttle vector rescue and analysis were performed as for Figure 3. (B) Triplex-induced recombination with a purine motif target. Cells pre-transfected with a dual supF plasmid containing a 30 bp G-rich site, pSupFAR, were transfected 1 day later with lipid reagent alone or with the indicated TFOs. Analysis of recombination events was performed as above. Amidate18 and G3pUmC18 bind with high affinity to the A-rich target site in the intergenic region in pSupFTC18 but not to the G-rich site in pSupFAR. AG30 binds well to the site in pSupFAR but not to that in pSupFTC18.