Table 2. Gap repair efficiency in exo1, mre11 and rad1 strains.

Relevant genotype	Transformants /µg ×104 uncut pSB110	pSB110a gap repair (×10–2)b	Fold increase	pSB110a gap repair (×10–2)b	Fold increase	Gene replacement (×10–3)c	Fold increase
EXO1 MRE11 RAD1	17.6 ± 2.8	24.3 ± 7.4		4.4 ± 1.3		8.2 ± 1.6
exo1Δ	16.2 ± 0.8	46.3 ± 9.6	1.9	9.8 ± 3.0	2.2	23.1 ± 2.9	2.8
mre11Δ	4.4 ± 1.8	7.4 ± 2.8	0.3	0.41 ± 0.1	0.09	21.9 ± 7.7	2.7
mre11-H125N	19.1 ± 6.5	18.4 ± 5.4	0.8	NDd		ND
rad1Δ	13.2 ± 3.9	16.0 ± 5.0	0.7	1.9 ± 0.8	0.4	2.6 ± 1.1	0.3
exo1Δ mre11Δ	0.9 ± 0.6	2.8 ± 1.6	0.1	0.08	0.02	ND
rad1Δ mre11Δ	3.3 ± 1.6	4.8 ± 1.6	0.2	0.26 ± 0.03	0.06	ND

^apSB110 is ARS⁺; pSB101 contains no ARS element.

^bGap repair frequencies were determined by the number of Ura⁺ Met⁺ transformants obtained from the gapped plasmid divided by the number of Ura⁺ Met⁺ transformants obtained from an equivalent amount of uncut pSB110 DNA.

^cGene replacement frequencies were determined by the number of Met⁺ transformants obtained from the MET17 fragment divided by the number of Met⁺ transformants obtained from an equivalent amount of uncut pSB110 DNA.

^dND, not determined.