Table 2. Strains and plasmids.
| Name | Relevant genotype, description | Reference, source | |
|---|---|---|---|
| Strains | JJC75a | lig7ts | B.Michel |
| 168 | trpC2 | C.Anagnostopoulos | |
| HVS610 | 168 insb pMAP149 in yerH | This work | |
| HVS609 | 168 ins pMAP52 in yerG | This work | |
| HVS611 | 168 ins pMAP59 in yerG, with plasmid pMAP141 | This work | |
| Plasmids | pDG148 | pBR322-pUB110 KanR lacI Pspac | (15) |
| pMUTIN2 | pBR322 lacZ lacI EmR Pspac | (16) | |
| pMAP52 | pMUTIN2 Pspac:(nt –20c to 275 of yerG) | This work | |
| pMAP59 | pMUTIN2MCS Pspac:(nt 330–666 of yerG) | This work | |
| pMAP65 | pUB110 KanR lacI | (9) | |
| pMAP138 | pDG148 Pspac:yoqV | This work | |
| pMAP140 | pDG148 Pspac:yerG | This work | |
| pMAP149 | pMUTIN2MCS Pspac:(nt 540–854 of yerH) | This work | |
| pIL253 | EmR | (17) | |
| pMAP139 | pMAP138Δ(pBR322 lacI) from AatII to PstI, KanR | This work | |
| pMAP141 | pMAP140Δ(pBR322 lacI) from AatII to PstI, KanR | This work |
aAB1157 background, hsdR thr+ pro+.
bins indicates the insertion of a given plasmid in the B.subtilis chromosome.
cGene numbering starts at the start codon.