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. 2005 Jun 15;19(12):1438–1443. doi: 10.1101/gad.1299305

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Figure 2. — Ink4a/Arf derepression upon Bmi1 loss prevents efficient Shh-induced CGNP proliferation. (A) qRT-PCR shows up-regulation of Ink4a and Arf mRNA expression in Bmi1^-/- CGNPs and cerebella. (B) Bmi1; (Cb) cerebellum. (B) Reduced BrdU incorporation in Bmi1^-/- CGNPs upon Shh treatment (p < 0.001) is rescued upon subsequent Arf loss (p < 0.01) but not by Arf heterozygosity. (C,D)In wild-type CGNPs, Shh treatment induces an increase in Bmi1 protein levels and a decrease in Ink4a (p < 0.005) and Arf (p < 0.02) mRNA expression. (Tub) Tubulin. (E) Reduced BrdU incorporation in Bmi1^-/- CGNPs upon Shh treatment is rescued by Ink4a/Arf loss (p < 0.05) and Ink4a/Arf heterozygosity (p < 0.03).