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Specific and cooperative coactivator function between PGC-1α and PRMT1. CV-1 cells were transiently transfected with MMTV(ERE)-LUC reporter plasmid (250 ng) and expression vectors encoding ERα (0.1 ng), PGC-1α (50 ng), and PRMT1, PRMT1(E153Q) mutant or CARM1 (250 ng). Luciferase activity was measured after growth in 20 nM estradiol. (Bottom of figure) Immunoblots of extracts from COS7 cells transfected with HA-tagged PRMT1 expression vectors were performed with antibodies against HA.
