Figure 3.

Methylation of PGC-1α by PRMT1. (A) Full-length GST-PGC-1α was incubated with GST-CARM1 or GST-PRMT1 and [³H]AdoMet for 1 h at 30°C. Products were analyzed by SDS-PAGE and fluorography. (B) Methylation of PGC-1α fragments was analyzed by SDS-PAGE (lower panels show Coomassie blue staining) and fluorography (upper panels). Diagram shows amino acid numbers. Asterisks show the stained fragments (lower panels) that were methylated (upper panels); positions of molecular weight markers are indicated beside the panels. (C) Mutant forms of the PGC-1α E fragment (sequence shown) were methylated by PRMT1 and analyzed by fluorography (left panel) and staining (right panel). In the R4K and R3K mutants, the bracketed Arg residues (R) were changed to Lys. The results presented are from a single experiment representative of three independent experiments.