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Role of methylated Arg residues of PGC-1α E region in coactivator function. (A) CV-1 cells were transfected with MMTV(ERE)-LUC reporter plasmid (250 ng) and plasmids encoding ERα (0.1 ng), PGC-1α wild type or R3K mutant (50 ng), and PRMT1 (200 ng) as indicated, and grown with 20 nM estradiol. (B) CV-1 cells were transfected with pERRα-Luc reporter plasmid (250 ng) and plasmids encoding PRMT1 (250 ng) and PGC-1α wild type or R3K mutant (50 ng).
