Figure 6.

Role of endogenous PRMT1 in PGC-1α coactivator function. COS7 cells in 24-well plates were transfected with 20, 40, or 60 pmol of two different siRNAs against PRMT1 (si#1 and si#2). Two days later, cells were transfected with pERRα-Luc reporter plasmid (125 ng), a PGC-1α expression vector (25 ng), and a Renilla luciferase reporter plasmid driven by a cytomegalovirus promoter (12.5 ng). Luciferase activities were quantified 48 h later; firefly luciferase activity was normalized by Renilla luciferase activity. Protein levels were determined by immunoblots from cell extracts of a fourth siRNA-transfected well. Results shown are from a single experiment that is representative of three independent experiments.