TABLE 3.
Homologous proteins of ORF46, ORF47, ORF48, ORF49, and ORF50 from phage TP901-1
| ORF | Function and alternative name | Size
|
Homologous protein of L. lactis phageb | % Identity (no. of aa)c | Reference | |
|---|---|---|---|---|---|---|
| aa | kDaa | |||||
| 46 | Distal tail protein; Dit | 253 | 29.1 (30) | ORF49 of phage Tuc2009 | 95 (253) | 55 |
| ORF253 structural protein of phage ul36 | 94 (253) | 39 | ||||
| 47 | Tail fiber; Tal | 918 | 102.1 (95 + 63*) | Tal2009 structural protein of phage Tuc2009 | 93 (918) | 38 |
| ORF908 of phage ul36 | 87 (920) | 39 | ||||
| ORF54 of phage bIL285 | 79 (545) | 14 | ||||
| Conserved domain in peptidase family | 2e-21d | |||||
| M23/M37 | ||||||
| 48 | Upper baseplate disc; BppU | 299 | 33.8 (35) | ORF51 of phage Tuc2009 | 80 (296) | 55 |
| ORF322 structural protein of phage ul36 | 79 (296) | 39 | ||||
| 49 | Lower baseplate disc; BppL | 163 | 17.2 (17) | ORF343 of L. lactis φLC3 | 70 (151) | 4 |
| ORF53 structural protein of phage Tuc2009 | 56 (62) | 55 | ||||
| ORF165B structural protein of phage ul36 | 33 (65) | 39 | ||||
| Receptor binding proteins of phages jw30, jw32, fd13, and jw31 | 28-26 (112) | 20, 21 | ||||
| 50 | Unknown | 74 | 8.7 (-)e | ORF74 of phage φLC3 | 98 (74) | 4 |
| Putative holin of phage BK5-T | 98 (73) | 49 | ||||
| ORF58 of phage bIL286 | 98 (74) | 14 | ||||
| ORF60 of phage bIL285 | 95 (74) | 14 | ||||
| ORF54 of phage Tuc2009 | 95 (74) | 55 | ||||
a
Predicted molecular weight from aa sequence and the experimentally measured molecular weight by SDS-PAGE in parentheses. *, processed protein.
b
Identified from BLASTp search (2).
c
The percentage identity followed by the number of amino acids (aa) over which the identity is determined in parentheses.
d
E value from BLAST conserved domain alignment.
e
Not observed by SDS-PAGE analysis.