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Map of pOR1 plasmid (IVET vector) used to identify P. putida root-activated promoters. Modifications to the original plasmid pIVPRO (20) are described in the text. This plasmid has the replication origin oriR6K and thus requires protein Π for stable maintenance. The plasmid is therefore a suicide in Pseudomonas and is mobilizable as tra functions are supplied in trans.
