FIG. 2.
Effect of rap promoter activity on colonization ability. (A) Survival of chromosomal IVET cointegrates pool in the rhizosphere. ○, KT2440R; •, Δasd; ▵, a pool of approximately 500 clones of P. putida IVET cointegrates. (B) Survival of rap fusion strains. ○, KT2440R; •, Δasd; ▵, rap1-2 fusion; and ▴, rap1-4 fusion. Maize seeds were inoculated and plants grown as described in Materials and Methods. Bacterial cells were recovered on plates at the indicated times. At time zero, CFU was per g of seed; afterwards, CFU is plotted per g of rhizosphere including root. Means and standard deviations for three independent experiments are shown.