FIG. 1.

Primer extension analysis of ytmI and ytlI RNA in wild-type and spx mutant cells. Cultures were grown in TSS medium containing either MgSO₄ or methionine (see Materials and Methods) to OD₆₀₀s of 0.7. RNA was harvested as described previously (35). Primer extension reactions were performed using oligonucleotides oSN04-90 (for ytlI) and oSN04-91 (for ytmI). Sanger dideoxynucleotide termination sequence reactions were performed using the same primers. Products were resolved by polyacrylamide-urea gel electrophoresis and visualized by phosphorimaging. WT, wild type (JH642); spx, spx mutant (ORB3834); s, sulfate; m, methionine. (A) Primer extension products of ytlI RNA. Sequencing reactions are shown on the left. (B) Primer extension products of ytmI RNA. At the bottoms of the primer extension product lanes are photographs of formaldehyde agarose gels of total RNA from each RNA sample.